The society transformation has resulted in tremendous changes in China''s social structure and interests, and the relationship-based trust has also changed a lot. Through in-depth empirical research, this paper reveals the structure and source of Guanxi trust and the impact on cooperation of enterprises. Results show that: (1) Active trust in relational trust is developed on the basis of passive trust. The establishment of corporate cooperative relationship mainly depends on active trust rather than passive trust. (2) Reputation is conducive to establish passive trust, which helps enterprises to choose best partners. Communication and reciprocity have a significant positive impact on active trust, and ultimately promote continuous cooperation among enterprises. The result of this paper not only enriches the Guanxi-based trust theory, but also provides a useful reference for enterprises to develop and improve trust relationship with partners in management practice.