Abstract:The low rate of technology transfer and the disconnect between industry, academia, and research supply and demand are structural bottlenecks currently hindering the in-depth advancement of my country’s innovation-driven development strategy. How to unclog technology transfer channels and activate collaborative innovation among enterprises through institutional innovation in technology market intermediaries is a core issue facing both theoretical research and policy practice. The establishment of the National Technology Transfer Center is an important institutional arrangement for the government to build a professional technology market intermediary system and address the disconnect between supply and demand of scientific and technological achievements. This paper uses A-share listed companies from 2010 to 2022 as a research sample. Using a quasi-natural experiment based on the establishment of the National Technology Transfer Center, it employs the difference-in-differences method to identify the impact and mechanism of technology market intermediaries on enterprise collaborative innovation. The study finds that the establishment of the National Technology Transfer Center significantly promotes enterprise collaborative innovation, a conclusion that remains valid after a series of robustness tests. Mechanism testing shows that the National Technology Transfer Center effectively plays its role as a technology market intermediary, positively impacting enterprise collaborative innovation through two paths: promoting knowledge spillover and driving resource integration. Further analysis reveals that the aforementioned facilitation effects are more pronounced in regions with more developed technology markets, enterprises with stronger basic R&D capabilities, and regions with higher flow of technology resources, revealing a three-pronged collaborative innovation promotion logic of “transferring out—receiving—smooth circulation.” This paper provides incremental evidence for local governments to further improve the technology market intermediary system and promote the transformation of scientific and technological achievements, and has important practical significance for optimizing the efficiency of innovation resource allocation and activating the vitality of collaborative innovation among enterprises.